London Regional Flow Cytometry Facility
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Welcome
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Main Contact Info
Kristin Chadwick
Robarts Research Institute / Western University
1151 Richmond St
London, ON N6A 5B7
Remittance Contact Info
Johanne Langford (519) 931-5777 ext 24178
Robarts Research Institute
1151 Richmond St
London, ON N6A 5B7
This facility has not published any Products. Please check back.
The following Products and Services are available within our facility:
Instruments
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FACSymphony A1 |
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CytoFLEX S |
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LSRIIThe LSR II (13 clr 2B-3V-5YG-3R) analysis cytometer can measure 13 fluorescent parameters from four spatially separated lasers: Blue (488nm/200 mW/2 PMTs), Yellow-green (561nm/50 mW/5 PMTs), Violet (405nm/50 mW/3 PMTs) and Red 642nm/75 mW/3 PMTs). |
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FACSCantoThe FACSCanto (10 clr 2B-4V/R-4YG) analysis cytometer can measure 10 fluorescent parameters from four lasers with three spatially separated beam paths: Blue (488nm/adjustable 200 mW/2 PMTs), Yellow-green (561nm/50 mW/4 PMTs), and colinear Violet (405nm/50 mW) and Red (642nm/75 mW) lasers (4 shared PMTs). |
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FACSAria IIIThe FACSAria III (13clr 2B-3V-5YG-3R) cell sorter can measure 13 fluorescent parameters from four spatially separated lasers: Blue (488nm/20 mW/2 PMTs), Yellow-green (561nm/50 mW/5 PMTs), Violet (405nm/30 mW/3 PMTs) and Red 642nm/17 mW/3 PMTs). Sort options include: (1) 1-4 populations into 5mL round bottom tubes or screw top microfuge tubes, (2) 1-2 populations into 15mL conical tubes, or (3) using the Automated Cell Deposition Unit (ACDU), defined numbers of cells can be sorted into multiwell plates or microscope slides (ideal for single cell cloning). Sorter is equipped with temperature control, and is housed in a biosafety cabinet to allow sorting of live, CL2 organisms. |
Computer Workstations for Data Analysis
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Mac Analysis Workstation (FlowJo X)iMac computer workstation with FlowJo X flow cytometry data analysis software. Microsoft Office (including Microsoft Word, PowerPoint and Excel) aids in data analysis & publishing. |
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